Hit picking antibodies

Use PipeBio's tools to find the most diverse sequences in your sample and hit-pick to express in vector and characterize your antibody sequences

Image showing phylogenetic tree on PipeBio with labels for sequence diversity, V gene and a data table with ELISA binding values

How to find the most diverse antibody sequences and hit pick ideal clones

Diversity selection and hit-picking with functional assay data such as ELISA and cell-sorting

Hit pick visually, based on diversity or apply powerful filters to find the best candidate sequences

  • Use PipeBio's diversity selection tool to extract the most diverse set of sequences
  • Define the clonotype on different regions, such as CDRs, FRs or entire VH or VL chains
  • Upload functional assay data and overlay with sequences to better hit pick optimal candidate sequences in your experiments
Table view of ELISA values highlighted with a conditional color scheme, amino acid barcharts of the CDR-H3 region and diversity scores for the antibody heavy chain sequences in the sample