Clonal hit expansion

Analyzing NGS or Sanger sequences from biopanning and phage display experiments on PipeBio

IgG antibodies clustered around characterized centroid sequence and a phylogenetic tree showing closely related sequences in antibody repertoire

Identifying similar clones

Clustering around Sanger sequences and picking developable NGS sequences

Clustering around centroid sequence

  • You can seed individual clusters from original hit antibodies that have shown target-specificity, in order to find closely related sequences with similar binding properties
  • Use your characterized antibody clones as a centroid sequence and cluster together with NGS repertoire sequences
  • Find related, developable sequences with less liabilities from the NGS repertoire with clustering and phylogenetic analysis