Analyzing NGS or Sanger sequences from biopanning and phage display experiments on PipeBio
Identifying similar clones
Clustering around Sanger sequences and picking developable NGS sequences
Clustering around centroid sequence
You can seed individual clusters from original hit antibodies that have shown target-specificity, in order to find closely related sequences with similar binding properties
Use your characterized antibody clones as a centroid sequence and cluster together with NGS repertoire sequences
Find related, developable sequences with less liabilities from the NGS repertoire with clustering and phylogenetic analysis
