Antibody hit expansion

Analyzing NGS or Sanger antibody sequences from biopanning and phage, yeast or mammalian display experiments on PipeBio

IgG antibodies clustered around characterized centroid sequence and a phylogenetic tree showing closely related sequences in antibody repertoire

Identifying clonal families and similar antibodies

Cluster around Sanger sequences and pick developable antibodies from NGS repertoire sequencing

Clustering around characterized hit antibody

  • You can seed individual clusters from original hit antibodies that have shown target-specificity, in order to find closely related sequences with similar binding properties
  • Use your characterized antibody clones as a centroid sequence and cluster together with NGS repertoire sequences
  • Find related, developable sequences with less liabilities from the NGS repertoire with clustering and phylogenetic analysis