Biopanning

Analyzing NGS or Sanger sequences from biopanning and phage display experiments on PipeBio

Identifying enriched sequences from biopanning

Steps for finding enriched clones between multiple biopanning rounds

Annotation and quality control

PipeBio’s annotation engine detects sequences with frameshifts, stop codons or missing regions
You can create summary reports of the imported and analyzed NGS samples
You can quickly filter the sequences in the Status dashboard and discard sequences with sequencing errors from subsequent analysis

Summary report of sequencing quality of all NGS biopanning samples on and status pie chart of one NGS sample showing correct and incorrect sequences and sequencing errors on PipeBio

Cluster and compare multiple samples

You can cluster across multiple regions and determine the amino acid identity for cluster sequence similarity
Use charts to see the largest clusters in the samples
Easily identify which sequence clusters are most enriched across biopanning rounds

Image showing clustering on CDR-H1, CDR-H2, CDR-H3 regions, a bar chart with the top 20 largest clusters and a plot with the 30 clusters with largest changes across biopanning rounds

Differentially enriched antibodies

Easily identify which antibody sequence clusters are most enriched across biopanning rounds on the Volcano Plot
PipeBio calculates the normalized fold change between panning rounds and allows sample comparison across multiple rounds to show statistically significant clonal enrichment (p-value threshold)
Visualize sample overlap and filter by clicking on interactive charts

Volcano plot showing most enriched antibody sequences in a biopanning experiment and a venn diagram showing NGS sample overlap from biopanning experiment on PipeBio bioinformatics platform

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