Phage Display

Use PipeBio to hit pick diverse annotated & quality controlled sequences from Phage Display panning rounds from fastq or fasta files.

Phage display panning is one of the most used laboratory techniques in antibody drug discovery. A central part of the discovery process is sequencing by either NGS or Sanger and subsequent analysis and combining with experimental assay data to identify candidates. The PipeBio Bioinformatics Cloud is tailored to support the entire process from data generation to final hit-picking.

Upload

Upload NGS and Sanger sequence data by simple drag and drop of your fastq or ab1 files to your desired project.
Upload NGS and Sanger sequence data by simple drag and drop of your fastq or ab1 files to your desired project.

Trim

Easily trim off region off primer or low-quality regions to avoid bias in results.
Easily trim off region off primer or low-quality regions to avoid bias in results.

Merge

Merge paired-end Illumia reads to increase sequence length to include entire variable domain of your antibody
Merge paired-end Illumia reads to increase sequence length to include entire variable domain of your antibody

Quality Control

Visually inspect NGS read quality by an aggregate quality plot of the entire data set and filter out potential short sequences.
Visually inspect NGS read quality by an aggregate quality plot of the entire data set and filter out potential short sequences.

Annotate

Annotate frameworks, CDR's and other regions of interest and report germline genes and mutations. All based on Kabat, IMGT or AHO numbering. De-risk antibody developability by detecting sequence liabilities.
Annotate frameworks, CDR’s and other regions of interest and report germline genes and mutations. All based on Kabat, IMGT or AHO numbering. De-risk antibody developability by detecting sequence liabilities.
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Cluster

Identify families of related sequences within your Phage Display library which may have similar functional characteristics or look for overexpressed antibodies.
Identify families of related sequences within your Phage Display library which may have similar functional characteristics or look for overexpressed antibodies.
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Add functional data

Add functional and assay data from external Excel files, such as ELISA and titers, to individual sequences for easy filtering to find best binding sequences.
Add functional and assay data from external Excel files, such as ELISA and titers, to individual sequences for easy filtering to find best binding sequences.

Hit-pick

Hit-pick the best clones from your phage display experiments based on criteria you define, based on functional data, sequence, similarity to known sequences etc. Label best binders for easy retrieval.
Hit-pick the best clones from your phage display experiments based on criteria you define, based on functional data, sequence, similarity to known sequences etc. Label best binders for easy retrieval.

Add to database

Store your best binders in a scalable database which will become a repository of sequence and assay data which can used in future humanization experiments etc.
Store your best binders in a scalable database which will become a repository of sequence and assay data which can used in future humanization experiments etc.
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Automate with Workflows

Run it all with a single click! Pipe all these steps together in a completely automated workflow to save time and lock down parameters for consistent data analysis across the entire team to ensure reproducible results.
Run it all with a single click! Pipe all these steps together in a completely automated workflow to save time and lock down parameters for consistent data analysis across the entire team to ensure reproducible results.
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Try PipeBio today!

Try it out on some of our sample data that is ready to go!