Hybridoma Analysis

Automate your entire analysis from raw NGS reads to paired VH/VL sequences

Single cell hybridoma sequencing and analysis has traditionally been a preferred option when working with full IgG antibodies requiring accurate paring of heavy and light chains. With next generation sequencing the challenge is to find dominant clones per sequencing well and spot contaminations and outliers when having hundreds of NGS fastq files to be analyzed.

Upload

Simply upload de-multiplexed fastq files from your plate based hybridoma sequencing run with simple drag and drop. The fastq files are typically paired-end reads to cover the entire variable domain of the antibody.
Simply upload de-multiplexed fastq files from your plate based hybridoma sequencing run with simple drag and drop. The fastq files are typically paired-end reads to cover the entire variable domain of the antibody.

Start workflow


<h3>Track progress</h3>



<figure class=The hybridoma workflow will pair reads, trim, annotate, cluster, and extract most dominant sequence per hybridoma. This may result in hundreds of individual processes which can easily be monitored.
The hybridoma workflow will pair reads, trim, annotate, cluster, and extract most dominant sequence per hybridoma. This may result in hundreds of individual processes which can easily be monitored.

Pair chains

 Pair VH/VL sequences from each well for further analysis or synthesis. If multiple candidate sequences are extracted per well will all combinations of VH/VL pairs be generated. Missing chains due to poor sequencing will also be handled gracefully.
Pair VH/VL sequences from each well for further analysis or synthesis. If multiple candidate sequences are extracted per well will all combinations of VH/VL pairs be generated. Missing chains due to poor sequencing will also be handled gracefully.

Add functional data to the antibody pair

Easily associate assay and functional data to each pair of VH/VL sequences. This can be ELISA, binding, titers etc.
Easily associate assay and functional data to each pair of VH/VL sequences. This can be ELISA, binding, titers etc.

Cluster

Cluster regions of individual VH or VL sequences or across the VH/VL pairs to find unique combinations of antibodies. This will allow to remove redundant data but also to find families of sequences which may have similar characteristics.
Cluster regions of individual VH or VL sequences or across the VH/VL pairs to find unique combinations of antibodies. This will allow to remove redundant data but also to find families of sequences which may have similar characteristics.

Align to see diversity

Visualize functional data, sequence diversity, clustering information in the alignment view. Alignment color scheme is adapted from VectorNTI.
Visualize functional data, sequence diversity, clustering information in the alignment view. Alignment color scheme is adapted from VectorNTI.

Continue your work

 Hit-pick your favorite VH/VL pairs based on information of clustering, associated assay data, visual inspection of phylogenetic tree and alignments, found liabilities etc. Sequences can also be sent to synthesis, the build-in database and a lot more.
Hit-pick your favorite VH/VL pairs based on information of clustering, associated assay data, visual inspection of phylogenetic tree and alignments, found liabilities etc. Sequences can also be sent to synthesis, the build-in database and a lot more.

Start today

Click here to try now or contact us and we will set up your own specialized and automated workflows.