An overview of Sanger tools and workflows on PipeBio Sanger sequencing has been routinely used to characterize immune cell populations and analyze antibody repertoires for more than twenty years. Despite the development of new NGS methods such as Illumina sequencing or microfluidic systems, Sanger sequencing still remains relevant and in wide use for its high… Continue reading Sanger sequencing analysis with PipeBio
Author: Max Gubert Olivé
Single-cell sequencing technologies in antibody research
Introduction With the current advancements in antibody drug discovery, there is an increasing demand for sequencing technologies to provide efficient tools able to characterize single immunological cells. Single-cell methods offer a deep insight into the complex biology of the immune system and enable the precise study of cell secretion, migration, cell-to-cell interaction and mRNA sequencing.… Continue reading Single-cell sequencing technologies in antibody research
PHAGE-ATAC: a novel approach for rapid nanobody generation
Many sequencing technologies have been developed to characterize the cell state including RNA-seq, ATAC-seq, and ChIP-seq. It has become highly desirable to apply multiple techniques to the same cell. PHAGE-ATAC runs single cell ATAC-seq and mitochondrial DNA sequencing while also detecting intracellular epitopes. One key application of PHAGE-ATAC is the rapid generation of antigen specific… Continue reading PHAGE-ATAC: a novel approach for rapid nanobody generation
Selecting antibodies with phage display technology
Introduction Phage display is a powerful technology widely used for identification and selection of peptides binding to a broad range of targets. Since its first demonstration in 1985, the method has been proven extremely successful in a variety of fields, such as protein engineering, drug discovery, medical diagnosis and therapeutic targeting. The principle of phage… Continue reading Selecting antibodies with phage display technology