Clonal hit expansion by mining antibodies in large NGS repertoires

How to use known target-specific antibodies to find related clones from next-generation repertoire sequencing data NGS is often used to explore the depths of the immune response against a given antigen as the cost of sequencing millions of antibodies is very low. The drawback is unfortunately that it is not possible and economically feasible to… Continue reading Clonal hit expansion by mining antibodies in large NGS repertoires

DNA synthesis in antibody discovery

An illustration of an antibody discovery workflow by repertoire sequencing, including gene synthesis-by PipeBio

Based on data from a subset of DNA synthesis service providers, error rates for DNA synthesis could range from 0.007% to 0.02%. Re-sequencing synthesized sequences before amplification and subsequent functional analysis can therefore be valuable for mitigating the risk for downstream errors. With the cost of synthesizing a base pair hovering around $0.10, the total… Continue reading DNA synthesis in antibody discovery